Template:Team:UC Berkeley/Notebook/MJV sequencing
From 2008.igem.org
(Difference between revisions)
(15 intermediate revisions not shown) | |||
Line 129: | Line 129: | ||
| MJV063 || 7-19-08 || mv16 || Clone #3 || ca998 || wrong part (a~pelB.rbs_ligase! instead of rbs_pelB.ligase!) || [[Team:UC Berkeley/Notebook/MJV/MJV063 | MJV063]] | | MJV063 || 7-19-08 || mv16 || Clone #3 || ca998 || wrong part (a~pelB.rbs_ligase! instead of rbs_pelB.ligase!) || [[Team:UC Berkeley/Notebook/MJV/MJV063 | MJV063]] | ||
|- | |- | ||
- | | MJV064 || 7-22-08 || mv1|| Clone #1 || ca998 || wrong part (doesn't even look like | + | | MJV064 || 7-22-08 || mv1|| Clone #1 || ca998 || wrong part (doesn't even look like it's in BBb) || [[Team:UC Berkeley/Notebook/MJV/MJV064 | MJV064]] |
|- | |- | ||
| MJV065 || 7-22-08 || mv10 || Clone #1 || ca998 || Perfect || [[Team:UC Berkeley/Notebook/MJV/MJV065 | MJV065]] | | MJV065 || 7-22-08 || mv10 || Clone #1 || ca998 || Perfect || [[Team:UC Berkeley/Notebook/MJV/MJV065 | MJV065]] | ||
Line 143: | Line 143: | ||
| MJV070 || 7-22-08 || ig1094 || Clone #2 || ca998 || wrong part (its {ihfB!}) || [[Team:UC Berkeley/Notebook/MJV/MJV070 | MJV070]] | | MJV070 || 7-22-08 || ig1094 || Clone #2 || ca998 || wrong part (its {ihfB!}) || [[Team:UC Berkeley/Notebook/MJV/MJV070 | MJV070]] | ||
|- | |- | ||
- | | MJV071 || 7-22-08 || ig1098 || Clone #6 || ca998 || wrong part (doesn't even look like | + | | MJV071 || 7-22-08 || ig1098 || Clone #6 || ca998 || wrong part (doesn't even look like it's in BBb) || [[Team:UC Berkeley/Notebook/MJV/MJV071 | MJV071]] |
|- | |- | ||
| MJV072 || 7-22-08 || ig1091 || Clone #1 || ca998 || Perfect partial || [[Team:UC Berkeley/Notebook/MJV/MJV072 | MJV072]] | | MJV072 || 7-22-08 || ig1091 || Clone #1 || ca998 || Perfect partial || [[Team:UC Berkeley/Notebook/MJV/MJV072 | MJV072]] | ||
Line 155: | Line 155: | ||
| MJV076 || 7-23-08 || ig114 || Clone #1 || ca998 || Bad read || [[Team:UC Berkeley/Notebook/MJV/MJV076 | MJV076]] | | MJV076 || 7-23-08 || ig114 || Clone #1 || ca998 || Bad read || [[Team:UC Berkeley/Notebook/MJV/MJV076 | MJV076]] | ||
|- | |- | ||
- | | MJV077 || 7-24-08 || mv4 || Clone #1 || ca998 || wrong part (doesn't even look like | + | | MJV077 || 7-24-08 || mv4 || Clone #1 || ca998 || wrong part (doesn't even look like it's in BBb) || [[Team:UC Berkeley/Notebook/MJV/MJV077 | MJV077]] |
|- | |- | ||
| MJV078 || 7-24-08 || mv7 || Clone #2 || ca998 || wrong part (rbs_BamHI part is missing) || [[Team:UC Berkeley/Notebook/MJV/MJV078 | MJV078]] | | MJV078 || 7-24-08 || mv7 || Clone #2 || ca998 || wrong part (rbs_BamHI part is missing) || [[Team:UC Berkeley/Notebook/MJV/MJV078 | MJV078]] | ||
Line 161: | Line 161: | ||
| MJV079 || 7-25-08 || ig1090 || Clone #1 || ca998 || perfect partial--insertion is an artifact of sequencing || [[Team:UC Berkeley/Notebook/MJV/MJV079 | MJV079]] | | MJV079 || 7-25-08 || ig1090 || Clone #1 || ca998 || perfect partial--insertion is an artifact of sequencing || [[Team:UC Berkeley/Notebook/MJV/MJV079 | MJV079]] | ||
|- | |- | ||
- | | MJV080 || 7-25-08 || ig117 || Clone #1 || ca998 || perfect | + | | MJV080 || 7-25-08 || ig117 || Clone #1 || ca998 || perfect partial || [[Team:UC Berkeley/Notebook/MJV/MJV080 | MJV080]] |
|- | |- | ||
| MJV081 || 7-25-08 || mv3 || Clone #2 || ca998 || perfect || [[Team:UC Berkeley/Notebook/MJV/MJV081 | MJV081]] | | MJV081 || 7-25-08 || mv3 || Clone #2 || ca998 || perfect || [[Team:UC Berkeley/Notebook/MJV/MJV081 | MJV081]] | ||
Line 171: | Line 171: | ||
| MJV076 || 7-25-08 || ig114 || Clone #2 || ca998 || perfect partial || [[Team:UC Berkeley/Notebook/MJV/MJV084 | MJV084]] | | MJV076 || 7-25-08 || ig114 || Clone #2 || ca998 || perfect partial || [[Team:UC Berkeley/Notebook/MJV/MJV084 | MJV084]] | ||
|- | |- | ||
- | | MJV085 || Date || Plasmid || Clone # || Oligo || Result || [[Team:UC Berkeley/Notebook/MJV/ | + | | MJV086 || 7-28-08 || mv1 || Clone #4 || ca998 || perfect || [[Team:UC Berkeley/Notebook/MJV/MJV085 | MJV085]] |
+ | |- | ||
+ | | MJV087 || 7-28-08 || ig1094 || Clone #4 || ca998 || perfect partial || [[Team:UC Berkeley/Notebook/MJV/MJV086 | MJV086]] | ||
+ | |- | ||
+ | | MJV087 || 7-28-08 || ig1094 || Clone #4 || g00101 || wrong sample? || [[Team:UC Berkeley/Notebook/MJV/MJV087 | MJV087]] | ||
+ | |- | ||
+ | | MJV088 || 7-28-08 || ig1098 || Clone #4 || ca998 || wrong part (it's {<Cre>}) || [[Team:UC Berkeley/Notebook/MJV/MJV088 | MJV088]] | ||
+ | |- | ||
+ | | MJV089 || 7-29-08 || ig1098 || Clone #7 || ca998 || wrong part (doesn't even look like it's BBb) || [[Team:UC Berkeley/Notebook/MJV/MJV089 | MJV089]] | ||
+ | |- | ||
+ | | MJV089 || 7-31-08 || ptet [C/K] || Clone #1 || ca998 || correct part, but deletion in the vector between BamHI site and BBb scar || [[Team:UC Berkeley/Notebook/MJV/MJV089_1 | MJV089]] | ||
+ | |- | ||
+ | | MJV090 || 7-31-08 || mv20 || Clone #1 || ca998 || perfect || [[Team:UC Berkeley/Notebook/MJV/MJV090 | MJV090]] | ||
+ | |- | ||
+ | | MJV091 || 8-1-08 || ptet [C/K] || Clone #2 || ca998 || correct part, but deletion in the vector between BamHI site and BBb scar || [[Team:UC Berkeley/Notebook/MJV/MJV091 | MJV091]] | ||
+ | |- | ||
+ | | MJV092 || 8-04-08 || mv16 [A/C] || Clone #1 || ca998 || Perfect Partial || [[Team:UC Berkeley/Notebook/MJV/MJV092 | MJV092]] | ||
+ | |- | ||
+ | | MJV093 || 8-04-08 || mv23 [C/A] || Clone #1 || ca998 || Perfect || [[Team:UC Berkeley/Notebook/MJV/MJV093 | MJV093]] | ||
+ | |- | ||
+ | | MJV094 || 8-05-08 || pBjh1600-K112120 || Clone #2 || ca998 || Perfect || [[Team:UC Berkeley/Notebook/MJV/MJV094 | MJV094]] | ||
+ | |- | ||
+ | | MJV095 || 8-05-08 || ig1094 || Clone #1 || ca998 || Perfect partial || [[Team:UC Berkeley/Notebook/MJV/MJV095 | MJV095]] | ||
+ | |- | ||
+ | | MJV096 || 8-05-08 || pBad[K/C] || Clone #1 || ca998 || Ok partial (has point mutations) || [[Team:UC Berkeley/Notebook/MJV/MJV096 | MJV096]] | ||
+ | |- | ||
+ | | MJV097 || 8-5-08 || mv10 [C/K] || Clone #2 || ca998 || Perfect || [[Team:UC Berkeley/Notebook/MJV/MJV097 | MJV097]] | ||
+ | |- | ||
+ | | MJV098 || 8-5-08 || mv15 [K/A] || Clone #5 || ca998 || missing BamHI part || [[Team:UC Berkeley/Notebook/MJV/MJV98 | MJV098]] | ||
+ | |- | ||
+ | | MJV099 || 8-7-08 || pBca1256-OriV || Clone #1 || ca998 || wrong part-something else from genome || [[Team:UC Berkeley/Notebook/MJV/MJV099 | MJV099]] | ||
+ | |- | ||
+ | | MJV100 || 8-7-08 || pBca1256-OriV || Clone #2 || ca998 || wrong part-something else from genome || [[Team:UC Berkeley/Notebook/MJV/MJV100 | MJV100]] | ||
+ | |- | ||
+ | | MJV101 || 8-7-08 || pBca1256-attR2 || Clone #1 || ca998 || same point mutation that Dan had || [[Team:UC Berkeley/Notebook/MJV/MJV101 | MJV101]] | ||
+ | |- | ||
+ | | MJV102 || Date || Plasmid || Clone # || Oligo || Result || [[Team:UC Berkeley/Notebook/MJV/MJV102 | MJV102]] | ||
|} | |} | ||
---- | ---- |
Latest revision as of 20:56, 26 August 2008
Tube ID | Date | Plasmid | Clone # | Oligo | Result | File |
MV_G3 | 6-20-08 | pBca1256-K112106 | Clone #1 | ca998 | Perfect | MJV001 |
MV_G1 | 6-20-08 | pBca1256-K112107 | Clone #1 | ca998 | Perfect | MJV002 |
MV_F6 | 6-20-08 | pBca1256-K112108 | Clone #1 | ca998 | Perfect | MJV003 |
MV_F5 | 6-20-08 | pBca1256-K112117 | Clone #1 | ca998 | Perfect | MJV004 |
MV_F4 | 6-20-08 | pBca1256-K112121 | Clone #1 | ca998 | Perfect | MJV005 |
MV_F3 | 6-20-08 | pBca1256-K112103 | Clone #1 | ca998 | Bad read | MJV006 |
MV_F2 | 6-20-08 | pBca1256-K112116 | Clone #1 | ca998 | Huge deletion | MJV007 |
MV_F1 | 6-20-08 | pBca1256-K112120 | Clone #1 | ca998 | Bad read | MJV008 |
DV_4 | 6-20-08 | pBca1256-K112100 | Clone #1 | ca998 | Perfect Partial | MJV009 |
MV_rrnB | 6-20-08 | pBca1256-K112101 | Clone #1 | ca998 | Perfect Partial | MJV010 |
MV_C3 | 6-20-08 | pBca1256-K112102 | Clone #1 | ca998 | Perfect Partial | MJV011 |
MV_H5 | 6-20-08 | pBca1256-K112115 | Clone #1 | ca998 | Perfect Partial | MJV012 |
MV_H4 | 6-20-08 | pBca1256-K112119 | Clone #1 | ca998 | Perfect Partial | MJV013 |
MV_H3 | 6-20-08 | pBca1256-K112105 | Clone #1 | ca998 | Point mutation (not silent) | MJV014 |
MV_H2 | 6-20-08 | pBca1256-K112118 | Clone #1 | ca998 | Perfect | MJV015 |
ig001 | 6-21-08 | pBca1256-K112109 | Clone #5 | ca998 | Perfect Partial | MJV016 |
ig001 | 6-21-08 | pBca1256-K112109 | Clone #5 | g00101 | Perfect Partial | MJV017 |
ig002 | 6-21-08 | pBca1256-K112109 | Clone #6 | ca998 | Point mutations | MJV018 |
ig3 | 6-21-08 | pBca1256-K112109 | Clone #7 | ca998 | Bad read | MJV019 |
ig5 | 6-21-08 | pBca1256-K112103 | Clone #2 | ca998 | 3 point mutations | MJV020 |
ig4 | 6-21-08 | pBca1256-K112104 | Clone #1 | ca998 | point mutation (not silent) | MJV021 |
ig6 | 6-21-08 | pBca1256-K112105 | Clone #2 | ca998 | Perfect | MJV022 |
ig7 | 6-21-08 | pBca1256-K112116 | Clone #2 | ca998 | point mutation (not silent) | MJV023 |
ig8 | 6-21-08 | pBca1256-K112120 | Clone #2 | ca998 | Bad read | MJV024 |
DV_4 | 6-21-08 | pBca1256-K112100 | Clone #1 | g00101 | Bad read | MJV025 |
MV_rrnB | 6-21-08 | pBca1256-K112101 | Clone #1 | g00101 | Wrong part | MJV026 |
MV_C3 | 6-21-08 | pBca1256-K112102 | Clone #1 | g00101 | Wrong Part | MJV027 |
MV_H5 | 6-21-08 | pBca1256-K112115 | Clone #1 | g00101 | Bad read | MJV028 |
MV_H4 | 6-21-08 | pBca1256-K112119 | Clone #1 | g00101 | Perfect Partial | MJV029 |
ig16 | 6-23-08 | pBca1256-K112103 | Clone #3 | ca998 | Point Mutation (not silent) | MJV030 |
ig17 | 6-23-08 | pBca1256-K112116 | Clone #3 | ca998 | Parent vector (P_tet promoter part) | MJV031 |
ig18 | 6-23-08 | pBca1256-K112100 | Clone #1 | g00101 | Perfect partial | MJV032 |
ig19 | 6-23-08 | pBca1256-K112101 | Clone #1 | g00101 | Perfect partial | MJV033 |
ig20 | 6-23-08 | pBca1256-K112102 | Clone #1 | g00101 | Perfect partial | MJV034 |
ig21 | 6-23-08 | pBca1256-K112115 | Clone #1 | g00101 | Perfect partial | MJV035 |
MJV036 | 6-25-08 | pBca1256-K112110 | Clone #1 | ca998 | Deletion-might be an artifact of sequencing | MJV036 |
MJV037 | 6-25-08 | pBca1256-K112111 | Clone #1 | ca998 | Perfect partial | MJV037 |
MJV038 | 6-25-08 | pBca1256-K112112 | Clone #1 | ca998 | Perfect partial | MJV038 |
MJV039 | 6-25-08 | pBca1256-K112113 | Clone #1 | ca998 | Perfect partial | MJV039 |
MJV040 | 6-25-08 | pBca1256-K112114 | Clone #1 | ca998 | Perfect partial | MJV040 |
MJV041 | 6-25-08 | pBca1256-K112103 | Clone #4 | ca998 | Perfect | MJV041 |
MJV042 | 6-25-08 | pBca1256-K112104 | Clone #2 | ca998 | Wrong part of plasmid-insert missing | MJV042 |
MJV043 | 6-25-08 | pBca1256-K112116 | Clone #4 | ca998 | Bad read | MJV043 |
MJV044 | 6-25-08 | pBca1256-K112122 | Clone #1 | ca998 | Perfect partial | MJV044 |
MJV045 | 6-25-08 | pBca1256-K112123 | Clone #1 | ca998 | Perfect | MJV045 |
MJV036 | 6-25-08 | pBca1256-K112110 | Clone #1 | g00101 | Perfect partial | MJV046 |
MJV037 | 6-25-08 | pBca1256-K112111 | Clone #1 | g00101 | Perfect partial | MJV047 |
MJV038 | 6-25-08 | pBca1256-K112112 | Clone #1 | g00101 | Perfect partial | MJV048 |
MJV039 | 6-25-08 | pBca1256-K112113 | Clone #1 | g00101 | Perfect partial | MJV049 |
MJV040 | 6-25-08 | pBca1256-K112114 | Clone #1 | g00101 | Perfect partial | MJV050 |
MJV051 | 6-26-08 | pBca1256-K112116 | Clone #5 | ca998 | Point mutation (not silent) | MJV051 |
MJV043 | 6-26-08 | pBca1256-K112116 | Clone #4 | ca998 | Bad read | MJV052 |
MJV044 | 6-27-08 | pBca1256-K112122 | Clone #1 | g00101 | Perfect partial | MJV053 |
MJV054 | 6-27-08 | pBca1256-K112110 | Clone #2 | ca998 | Perfect partial | MJV054 |
MJV054 | 6-27-08 | pBca1256-K112110 | Clone #2 | g00101 | Perfect partial | MJV055 |
MJV056 | 6-30-08 | pBjh1600-K112104 | Clone #1 | ca998 | Perfect | MJV056 |
MJV057 | 6-30-08 | pBjh1600-K112104 | Clone #2 | ca998 | Perfect | MJV057 |
MJV058 | 6-30-08 | pBjh1600-K112116 | Clone #1 | ca998 | Bad Read | MJV058 |
MJV059 | 6-30-08 | pBjh1600-K112116 | Clone #3 | ca998 | Perfect | MJV059 |
MJV060 | 6-30-08 | pBjh1600-K112120 | Clone #2 | ca998 | Perfect | MJV060 |
MJV061 | 6-30-08 | pBjh1600-K112120 | Clone #3 | ca998 | Bad Read | MJV061 |
MJV062 | 7-19-08 | mv15 | Clone #2 | ca998 | wrong part (missing BamHI from rbs_phoApp.BamHI!) | MJV062 |
MJV063 | 7-19-08 | mv16 | Clone #3 | ca998 | wrong part (a~pelB.rbs_ligase! instead of rbs_pelB.ligase!) | MJV063 |
MJV064 | 7-22-08 | mv1 | Clone #1 | ca998 | wrong part (doesn't even look like it's in BBb) | MJV064 |
MJV065 | 7-22-08 | mv10 | Clone #1 | ca998 | Perfect | MJV065 |
MJV066 | 7-22-08 | mv10 | Clone #3 | ca998 | Bad read | MJV066 |
MJV067 | 7-22-08 | mv11 | Clone #6 | ca998 | Perfect partial | MJV067 |
MJV068 | 7-22-08 | mv11 | Clone #8 | ca998 | Perfect partial | MJV068 |
MJV069 | 7-22-08 | ig161 | Clone #2 | ca998 | Perfect | MJV069 |
MJV070 | 7-22-08 | ig1094 | Clone #2 | ca998 | wrong part (its {ihfB!}) | MJV070 |
MJV071 | 7-22-08 | ig1098 | Clone #6 | ca998 | wrong part (doesn't even look like it's in BBb) | MJV071 |
MJV072 | 7-22-08 | ig1091 | Clone #1 | ca998 | Perfect partial | MJV072 |
MJV072 | 7-22-08 | ig1091 | Clone #1 | g00101 | Perfect partial | MJV073 |
MJV074 | Date | Plasmid | Clone # | Oligo | Result | MJV074 |
MJV075 | 7-23-08 | ig1124 | Clone #1 | ca998 | Perfect | MJV075 |
MJV076 | 7-23-08 | ig114 | Clone #1 | ca998 | Bad read | MJV076 |
MJV077 | 7-24-08 | mv4 | Clone #1 | ca998 | wrong part (doesn't even look like it's in BBb) | MJV077 |
MJV078 | 7-24-08 | mv7 | Clone #2 | ca998 | wrong part (rbs_BamHI part is missing) | MJV078 |
MJV079 | 7-25-08 | ig1090 | Clone #1 | ca998 | perfect partial--insertion is an artifact of sequencing | MJV079 |
MJV080 | 7-25-08 | ig117 | Clone #1 | ca998 | perfect partial | MJV080 |
MJV081 | 7-25-08 | mv3 | Clone #2 | ca998 | perfect | MJV081 |
MJV078 | 7-24-08 | mv7 | Clone #2 | g00101 | perfect partial | MJV082 |
MJV079 | 7-25-08 | ig1090 | Clone #1 | g00101 | perfect partial | MJV083 |
MJV076 | 7-25-08 | ig114 | Clone #2 | ca998 | perfect partial | MJV084 |
MJV086 | 7-28-08 | mv1 | Clone #4 | ca998 | perfect | MJV085 |
MJV087 | 7-28-08 | ig1094 | Clone #4 | ca998 | perfect partial | MJV086 |
MJV087 | 7-28-08 | ig1094 | Clone #4 | g00101 | wrong sample? | MJV087 |
MJV088 | 7-28-08 | ig1098 | Clone #4 | ca998 | wrong part (it's {<Cre>}) | MJV088 |
MJV089 | 7-29-08 | ig1098 | Clone #7 | ca998 | wrong part (doesn't even look like it's BBb) | MJV089 |
MJV089 | 7-31-08 | ptet [C/K] | Clone #1 | ca998 | correct part, but deletion in the vector between BamHI site and BBb scar | MJV089 |
MJV090 | 7-31-08 | mv20 | Clone #1 | ca998 | perfect | MJV090 |
MJV091 | 8-1-08 | ptet [C/K] | Clone #2 | ca998 | correct part, but deletion in the vector between BamHI site and BBb scar | MJV091 |
MJV092 | 8-04-08 | mv16 [A/C] | Clone #1 | ca998 | Perfect Partial | MJV092 |
MJV093 | 8-04-08 | mv23 [C/A] | Clone #1 | ca998 | Perfect | MJV093 |
MJV094 | 8-05-08 | pBjh1600-K112120 | Clone #2 | ca998 | Perfect | MJV094 |
MJV095 | 8-05-08 | ig1094 | Clone #1 | ca998 | Perfect partial | MJV095 |
MJV096 | 8-05-08 | pBad[K/C] | Clone #1 | ca998 | Ok partial (has point mutations) | MJV096 |
MJV097 | 8-5-08 | mv10 [C/K] | Clone #2 | ca998 | Perfect | MJV097 |
MJV098 | 8-5-08 | mv15 [K/A] | Clone #5 | ca998 | missing BamHI part | MJV098 |
MJV099 | 8-7-08 | pBca1256-OriV | Clone #1 | ca998 | wrong part-something else from genome | MJV099 |
MJV100 | 8-7-08 | pBca1256-OriV | Clone #2 | ca998 | wrong part-something else from genome | MJV100 |
MJV101 | 8-7-08 | pBca1256-attR2 | Clone #1 | ca998 | same point mutation that Dan had | MJV101 |
MJV102 | Date | Plasmid | Clone # | Oligo | Result | MJV102 |