User:University of Washington/27 August 2008

From 2008.igem.org

(Difference between revisions)
(→LuxR from AraC and TetR(Faifan))
 
(One intermediate revision not shown)
Line 1: Line 1:
==LuxR from AraC and TetR(Faifan)==
==LuxR from AraC and TetR(Faifan)==
-
-
+
<h4>3A ligation</h4>
 +
-miniprepped x 6
 +
 +
-submit forward sequencing x 6
 +
 +
<h4>with CIP reaction</h4>
 +
 +
- ran gel on digested promoter, GFP, and LuxR and cut bands(3184 bp for promoter vector, 898 bp for GFP insert, and 958 bp for LuxR insert)
 +
 +
- did gel purification
 +
 +
- nanodropped at 260 nm
 +
<table border=1>
 +
<tr align="center">
 +
<th></th><th>ng/ul</th><th>260/280</th><th>260/230</th>
 +
</tr>
 +
<tr align="center">
 +
<td>promoter vector</td><td>129.9</td><td>1.86</td><td>0.61</td>
 +
</tr>
 +
<tr align="center">
 +
<td>GFP</td><td>20.8</td><td>2.35</td><td>0.04</td>
 +
</tr>
 +
<tr align="center">
 +
<td>LuxR</td><td>11.6</td><td>1.98</td><td>0.05</td>
 +
</tr>
 +
</table>
----
----
Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Latest revision as of 23:17, 27 August 2008

LuxR from AraC and TetR(Faifan)

3A ligation

-miniprepped x 6

-submit forward sequencing x 6

with CIP reaction

- ran gel on digested promoter, GFP, and LuxR and cut bands(3184 bp for promoter vector, 898 bp for GFP insert, and 958 bp for LuxR insert)

- did gel purification

- nanodropped at 260 nm

ng/ul260/280260/230
promoter vector129.91.860.61
GFP20.82.350.04
LuxR11.61.980.05

Back to Team:University_of_Washington/Notebook#Notebook

Retrieved from "http://2008.igem.org/User:University_of_Washington/27_August_2008"