User:University of Washington/27 August 2008

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(Difference between revisions)
(→LuxR from AraC and TetR(Faifan))
(→LuxR from AraC and TetR(Faifan))
 
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- nanodropped at 260 nm
- nanodropped at 260 nm
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<table border=1>
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<tr align="center">
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<th></th><th>ng/ul</th><th>260/280</th><th>260/230</th>
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</tr>
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<tr align="center">
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<td>promoter vector</td><td>129.9</td><td>1.86</td><td>0.61</td>
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</tr>
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<tr align="center">
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<td>GFP</td><td>20.8</td><td>2.35</td><td>0.04</td>
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</tr>
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<tr align="center">
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<td>LuxR</td><td>11.6</td><td>1.98</td><td>0.05</td>
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</tr>
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</table>
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Latest revision as of 23:17, 27 August 2008

LuxR from AraC and TetR(Faifan)

3A ligation

-miniprepped x 6

-submit forward sequencing x 6

with CIP reaction

- ran gel on digested promoter, GFP, and LuxR and cut bands(3184 bp for promoter vector, 898 bp for GFP insert, and 958 bp for LuxR insert)

- did gel purification

- nanodropped at 260 nm

ng/ul260/280260/230
promoter vector129.91.860.61
GFP20.82.350.04
LuxR11.61.980.05

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