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Template:Team:UC Berkeley/Notebook/MJV construction
From 2008.igem.org
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''Construction of <Cre! Basic Part K112100'' | ''Construction of <Cre! Basic Part K112100'' | ||
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PCR mv001F/mvoo1R on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) | PCR mv001F/mvoo1R on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) | ||
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Revision as of 21:49, 4 June 2008
Construction of <Cre! Basic Part K112100
PCR mv001F/mvoo1R on pSB1A2-I13521 (1261bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112800 ----------------------------------------------- ca8373 Forward Biobricking of mRFP CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag ca8374 Reverse Biobricking of mRFP CGttaGGATCCttactcccccaagaaaatcc
