Template:Team:UC Berkeley/Notebook/MJV construction

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'''Construction of <Cre! Basic Part K112100'''
'''Construction of <Cre! Basic Part K112100'''
<pre>
<pre>
-
PCR mv001F/mvoo1R on pSB1A2-I13521    (1261bp, EcoRI/BamHI/DpnI)
+
PCR mv001/mv002 on pBca9145-Bca1117    (1060bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
-
Product is pBca1256-K112800
+
Product is pBca1256-K112100
-----------------------------------------------
-----------------------------------------------
-
ca8373 Forward Biobricking of mRFP
+
mv001 Forward Biobricking of <Cre!
-
CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
+
ggataGAATTCgtgAGATCTtccaatttactgaccgtacac
-
ca8374 Reverse Biobricking of mRFP
+
mv002 Reverse Biobricking of <Cre!
-
CGttaGGATCCttactcccccaagaaaatcc
+
cgttaGGATCCttaatcgccatcttccagcaggcg
</pre>
</pre>

Revision as of 22:29, 4 June 2008

Construction of <Cre! Basic Part K112100 

PCR mv001/mv002 on pBca9145-Bca1117    (1060bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112100
-----------------------------------------------
mv001	Forward Biobricking of <Cre!
ggataGAATTCgtgAGATCTtccaatttactgaccgtacac
mv002	Reverse Biobricking of <Cre!
cgttaGGATCCttaatcgccatcttccagcaggcg
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