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Template:Team:UC Berkeley/Notebook/BX construction
From 2008.igem.org
(Difference between revisions)
(→Sixpi 22:25, 4 June 2008 (UTC)) |
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bx4 Reverse Biobricking of {<T4 Holin>} | bx4 Reverse Biobricking of {<T4 Holin>} | ||
cgttaGGATCCtttagcccttcctaatattctggcc | cgttaGGATCCtttagcccttcctaatattctggcc | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | PCR bx3/bx4 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112004 | ||
| + | ---------------------------------------- | ||
| + | bx5 Forward Biobricking of {a~T4 Holin!} | ||
| + | CCATAGAATTCatgAGATCTgtctatggcagcacctagaat | ||
| + | bx2 Reverse Biobricking of {a~T4 Holin!} | ||
| + | cgttaGGATCCttatttagcccttcctaatattct | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | PCR bx6/bx2 on Bjh1301 (701 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI) | ||
| + | Product is pBca1256-K112005 | ||
| + | ---------------------------------------- | ||
| + | bx6 Forward Biobricking of {rbs.T4 Holin!} | ||
| + | CCATAGAATTCatgAGATCTgaagaaaaaacatatggcagcacctagaat | ||
| + | bx2 Reverse Biobricking of {rbs.T4 Holin!} | ||
| + | cgttaGGATCCttatttagcccttcctaatattct | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | |||
</pre> | </pre> | ||
Revision as of 22:49, 4 June 2008
Sixpi 22:25, 4 June 2008 (UTC)
PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112000
----------------------------------------
bx1 Forward Biobricking of {T4 Holin!}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx2 Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3 Forward biobricking of {<T4 Holin!}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx2 Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattc
PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1 Forward biobricking of {T4 Holin>}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx4 Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3 Forward Biobricking of {<T4 Holin>}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx4 Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
PCR bx3/bx4 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112004
----------------------------------------
bx5 Forward Biobricking of {a~T4 Holin!}
CCATAGAATTCatgAGATCTgtctatggcagcacctagaat
bx2 Reverse Biobricking of {a~T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx6/bx2 on Bjh1301 (701 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6 Forward Biobricking of {rbs.T4 Holin!}
CCATAGAATTCatgAGATCTgaagaaaaaacatatggcagcacctagaat
bx2 Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
