Team:Hawaii/Notebook/2008-06- 3

(Difference between revisions)

Latest revision as of 02:34, 5 June 2008

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Grace & Norman

**Cell Count**
Dilution	Colony Forming Units on Plate	Number of Cells per mL
1	Too many to count	n/a
1/10	Too many to count	n/a
1/100	Too many to count	n/a
1/1000	190	19,000,000
1/10,000	40	4,000,000
1/100,000	23	23,000,000
1/1,000,000	6	600,000

DH5-α dilutions plated on LB

We have lots of cells (yay!). Cryofreezing will result in the loss cell population, but we will very likely still have viable cells after thawing.

"Wow, competency takes a long time." - NW

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 6: / Line 6: @@
 * Thaw test for PCC6803 and DH5-&alpha;
 * Made LB and LB + amp media
+:* amp conc. = 100 &mu;g/mL
 * Made 1000X amp stock solution, stored in 4C freezer
+:* 1000X = 100 mg/mL
 * Tested competency of DH5-&alpha; [[Team:Hawaii/Test_Competent_E._Coli | (see experiment)]]
 * Counted colonies to determine number of cells grown to OD600 = 0.3
@@ Line 19: / Line 21: @@
 |-
 ! 1
-| Too many to count || n/a
+| align="center"|Too many to count || align="center"|n/a
 |-
 ! 1/10
-|Too many to count
+|align="center"|Too many to count
-|n/a
+|align="center"|n/a
 |-
 ! 1/100
-| Too many to count|| n/a
+| align="center"|Too many to count|| align="center"|n/a
 |-
 ! 1/1000
-| 190|| 19,000,000
+| align="center"|190|| align="center"|19,000,000
 |-
 ! 1/10,000
-| 40|| 4,000,000
+| align="center"|40|| align="center"|4,000,000
 |-
 ! 1/100,000
-| 23|| 23,000,000
+| align="center"|23|| align="center"|23,000,000
 |-
 ! 1/1,000,000
-| 6|| 60,000
+| align="center"|6|| align="center"|600,000
 |}
 [[Image:DSC00696.JPG|left|thumb|250px|DH5-&alpha; dilutions plated on LB]]
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 == Discussion ==