Team:University of Lethbridge/Notebook/GeneralLabSeptember

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[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
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===August 31, 2008===
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====Nathan Puhl, Roxanne====
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-Transformed DH5a cells with the biobrick part R0011 (pLacI) obtained from the 2007 iGEM parts. Plated on LB + amp agar plates.
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===September 1, 2008===
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====Roxanne====
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-Picked 3 representative colonies and incubated them in LB+amp media overnight at 37.0C.
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===September 2, 2008===
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====Roxanne====
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-plasmid prepped the pLacI cells which had been incubated the night before. Ran plasmids on a 1% Agarose Gel at 100V for 27 minutes
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====Nathan Puhl====
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-Visualized the Gel and Glycerol Stocked the Cells.
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===September 3, 2008===
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====Roxanne, Nathan Puhl====
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-Performed a Restriction Digest of I13504 (RBS+GFP+dT) and R0011 (pLacI recombinant)
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-10 uL template DNA
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-0.5 uL Restriction Enzyme #1
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-0.5 uL Restriction Enzyme #2
 +
-2 uL NEB 2
 +
-7 uL ddH2O
 +
_________
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20 uL Reaction overnight at 37.0C

Revision as of 23:44, 3 September 2008

Back to The University of Lethbridge Main Notebook

Contents

August 31, 2008

Nathan Puhl, Roxanne

-Transformed DH5a cells with the biobrick part R0011 (pLacI) obtained from the 2007 iGEM parts. Plated on LB + amp agar plates.

September 1, 2008

Roxanne

-Picked 3 representative colonies and incubated them in LB+amp media overnight at 37.0C.

September 2, 2008

Roxanne

-plasmid prepped the pLacI cells which had been incubated the night before. Ran plasmids on a 1% Agarose Gel at 100V for 27 minutes

Nathan Puhl

-Visualized the Gel and Glycerol Stocked the Cells.

September 3, 2008

Roxanne, Nathan Puhl

-Performed a Restriction Digest of I13504 (RBS+GFP+dT) and R0011 (pLacI recombinant) 

-10 uL template DNA
-0.5 uL Restriction Enzyme #1
-0.5 uL Restriction Enzyme #2
-2 uL NEB 2
-7 uL ddH2O
_________
20 uL Reaction overnight at 37.0C
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