"
Team:Paris/Modeling/f1
From 2008.igem.org
(Difference between revisions)
| Line 28: | Line 28: | ||
|[expr(pTet)] | |[expr(pTet)] | ||
|expression rate of <br> pTet '''with RBS E0032''' | |expression rate of <br> pTet '''with RBS E0032''' | ||
| - | |nM. | + | |nM.min<sup>-1</sup> |
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures | |see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures | ||
|- | |- | ||
|γ<sub>GFP</sub> | |γ<sub>GFP</sub> | ||
|dilution-degradation rate <br> of GFP(mut3b) | |dilution-degradation rate <br> of GFP(mut3b) | ||
| - | | | + | |min<sup>-1</sup> |
| - | | | + | |0.0198 |
|- | |- | ||
|[GFP] | |[GFP] | ||
| Line 62: | Line 62: | ||
|β<sub>tet</sub> | |β<sub>tet</sub> | ||
|production rate of pTet '''with RBS E0032''' <br> β<sub>1</sub> | |production rate of pTet '''with RBS E0032''' <br> β<sub>1</sub> | ||
| - | |nM. | + | |nM.min<sup>-1</sup> |
| | | | ||
|- | |- | ||
|(K<sub>tet</sub>/{coef<sub>tetR</sub>}<sup>n<sub>tet</sub></sup>) | |(K<sub>tet</sub>/{coef<sub>tetR</sub>}<sup>n<sub>tet</sub></sup>) | ||
|activation constant of pTet <br> K<sub>18</sub> | |activation constant of pTet <br> K<sub>18</sub> | ||
| - | |nM | + | |nM |
| | | | ||
|- | |- | ||
| Line 77: | Line 77: | ||
|K<sub>aTc</sub> | |K<sub>aTc</sub> | ||
|complexation constant aTc-TetR <br> K<sub>17</sub> | |complexation constant aTc-TetR <br> K<sub>17</sub> | ||
| - | |nM | + | |nM |
| | | | ||
|- | |- | ||
Revision as of 22:13, 4 September 2008
so, with
that gives, after reducing the expression
with the previous expression of [TetR]eq
so, finally, if we use a calculated value of [TetR] (by using the expression of ƒ0 for instance, or by introducing a value given by tetR after an other "caracterised promoter")
| param | signification | unit | value |
| [expr(pTet)] | expression rate of pTet with RBS E0032 | nM.min-1 | see "findparam" need for 20 measures |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 measures |
| (fluorescence) | value of the observed fluorescence | au | need for 20 measures |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| βtet | production rate of pTet with RBS E0032 β1 | nM.min-1 | |
| (Ktet/{coeftetR}ntet) | activation constant of pTet K18 | nM | |
| ntet | complexation order of pTet n18 | no dimension | |
| KaTc | complexation constant aTc-TetR K17 | nM | |
| naTc | complexation order aTc-TetR n17 | no dimension |
