"
Team:University of Lethbridge/Notebook/Project4September
From 2008.igem.org
(Difference between revisions)
m |
m (→September 8, 2008) |
||
| Line 3: | Line 3: | ||
===September 7, 2008=== | ===September 7, 2008=== | ||
====Selina==== | ====Selina==== | ||
| + | Objective: Create glycerol stocks of the xylE pVL1392 plasmid for future use. | ||
| + | |||
Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells. | Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells. | ||
| Line 8: | Line 10: | ||
Plated 100 uL on LB + Amp and incubated overnight at 37C. | Plated 100 uL on LB + Amp and incubated overnight at 37C. | ||
| + | |||
| + | |||
| + | ===September 8, 2008=== | ||
| + | ====Selina==== | ||
| + | |||
| + | Checked xylE transformation plates for colonies - success! | ||
| + | |||
| + | Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours. | ||
| + | |||
| + | |||
| + | ====Christa, Selina==== | ||
| + | |||
| + | Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C. | ||
Revision as of 22:06, 8 September 2008
Back to The University of Lethbridge Main Notebook
Contents |
September 7, 2008
Selina
Objective: Create glycerol stocks of the xylE pVL1392 plasmid for future use.
Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells.
Followed manufacturer's (NEB) 'high-yield' protocol.
Plated 100 uL on LB + Amp and incubated overnight at 37C.
September 8, 2008
Selina
Checked xylE transformation plates for colonies - success!
Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours.
Christa, Selina
Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C.
