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Team:Warsaw
From 2008.igem.org
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<p>We want to note that our initial plans were very different from what we actually managed to do. Our plans were really amazing: we wanted to create system of biological machines which would allow to investigate protein interactions and simultaneously change sequence of one of them in order to achieve the strongest possible interaction. To make it work we planned to put the DNA sequence of tested protein on low-copy plasmid and transform it to bacterial strain in which it would be mutated (lets call the strain ‘slot machine’). Parts present on this plasmid would cause the protein to be attached to outer bacterial membrane, where the selection would occur. We planned that such selection system would allow us to search for antibodies with new specificities or screen protein libraries. </p></td> | <p>We want to note that our initial plans were very different from what we actually managed to do. Our plans were really amazing: we wanted to create system of biological machines which would allow to investigate protein interactions and simultaneously change sequence of one of them in order to achieve the strongest possible interaction. To make it work we planned to put the DNA sequence of tested protein on low-copy plasmid and transform it to bacterial strain in which it would be mutated (lets call the strain ‘slot machine’). Parts present on this plasmid would cause the protein to be attached to outer bacterial membrane, where the selection would occur. We planned that such selection system would allow us to search for antibodies with new specificities or screen protein libraries. </p></td> | ||
<td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" /></td> | <td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" /></td> | ||
| + | </tr> | ||
| + | <tr><td> | ||
| + | <h2>Institutions whom we would like to help for financial and technical support:</h2> | ||
| + | <table width="100%" border="0" cellspacing="10" cellpadding="0" align="center"> | ||
| + | <tr> | ||
| + | <td align="center" valign="bottom"><a href="http://www.uw.edu.pl/en/"><img src="https://static.igem.org/mediawiki/2008/6/67/Uw.gif" width="150" height="148" border="0" /></a> | ||
| + | <a href="http://www.uw.edu.pl/en/"><h3>University of Warsaw</h3> </a></td> | ||
| + | <td align="center" valign="bottom"><a href="http://www.fundacjauv.org.pl/"><img src="https://static.igem.org/mediawiki/2008/4/4d/Fundacja_uv-1.gif" width="160" height="65" border="0" /></a> | ||
| + | <a href="http://www.fundacjauv.org.pl/"><h3>The Universitatis Varsoviensis Fund </h3></a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/"><img src="https://static.igem.org/mediawiki/2008/f/f3/Faculty-of-Biology.gif" width="91" height="120" border="0" /></a> | ||
| + | <a href="http://www.biol.uw.edu.pl/"><h3>Faculty of Biology, University of Warsaw </h3></a> </td> | ||
| + | <td align="center" valign="bottom"><a href="http://www.fnp.org.pl/ang/index.html"><img src="https://static.igem.org/mediawiki/2008/c/c9/LogoFNP1wers.gif" width="312" height="106" border="0" /></a> | ||
| + | <a href="http://www.fnp.org.pl/ang/index.html"><h3>Foundation for Polish Science </h3></a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td align="center" valign="bottom"><a href="http://oligo.pl/English/"><img src="https://static.igem.org/mediawiki/2008/8/8e/Oligo.gif" width="183" height="64" border="0" /></a> </td> | ||
| + | <td align="center" valign="bottom"><h3>Rada Konsultacyjna ds. Studenckiego Ruchu Naukowego</h3></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=529&b_2501=5"><h3>Department of Bacterial Genetics </h3></a></td> | ||
| + | <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=526&b_2501=5"><h3>Department of Virology</h3> </a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=541&b_2501=5"><h3>Department of Plant Molecular Biology </h3></a></td> | ||
| + | <td align="center" valign="bottom"><a href="http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=534&b_2501=5"><h3>Institute of Genetics and Biotechnology</h3></a> </td> | ||
</tr> | </tr> | ||
| + | </table> | ||
| + | </td> | ||
| + | <td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" /> | ||
| + | </td> | ||
| + | </tr> | ||
</table> | </table> | ||
</html> | </html> | ||
Revision as of 08:57, 11 September 2008
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 We want to note that our initial plans were very different from what we actually managed to do. Our plans were really amazing: we wanted to create system of biological machines which would allow to investigate protein interactions and simultaneously change sequence of one of them in order to achieve the strongest possible interaction. To make it work we planned to put the DNA sequence of tested protein on low-copy plasmid and transform it to bacterial strain in which it would be mutated (lets call the strain ‘slot machine’). Parts present on this plasmid would cause the protein to be attached to outer bacterial membrane, where the selection would occur. We planned that such selection system would allow us to search for antibodies with new specificities or screen protein libraries. |
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Institutions whom we would like to help for financial and technical support: |
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