Alberta NINT/17 May 2008

From 2008.igem.org

(Difference between revisions)
(New page: lab work (SD, JD): PCR of T/A I oligonucleotide using Term + and Term - primers. DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for insertion int...)
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lab work (SD, JD): PCR of T/A I oligonucleotide using Term + and Term - primers.  DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for insertion into plasmid.
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lab work (SD, JD):  
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              PCR of T/A I oligonucleotide using Term + and Term - primers  
 +
              DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for insertion into plasmid.

Revision as of 21:34, 6 June 2008

lab work (SD, JD): 

             PCR of T/A I oligonucleotide using Term + and Term - primers 
             DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for insertion into plasmid.
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