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Frozen glycerol stock
From 2008.igem.org
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Riachalder (Talk | contribs) (New page: * Take 2mls of ON culture and pipette into a sterilised cryo tube. * Add 0.5ml of autoclaved 50% glycerol solution * Vortex briefly to mix * Store at -80˚C) |
Riachalder (Talk | contribs) |
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| + | ==Making Frozen Glycerol Stock from Overnight Culture== | ||
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* Take 2mls of ON culture and pipette into a sterilised cryo tube. | * Take 2mls of ON culture and pipette into a sterilised cryo tube. | ||
* Add 0.5ml of autoclaved 50% glycerol solution | * Add 0.5ml of autoclaved 50% glycerol solution | ||
* Vortex briefly to mix | * Vortex briefly to mix | ||
* Store at -80˚C | * Store at -80˚C | ||
| + | |||
| + | Back to [[Team:Newcastle University/Notebook]] | ||
Latest revision as of 11:44, 18 September 2008
Making Frozen Glycerol Stock from Overnight Culture
- Take 2mls of ON culture and pipette into a sterilised cryo tube.
- Add 0.5ml of autoclaved 50% glycerol solution
- Vortex briefly to mix
- Store at -80˚C
Back to Team:Newcastle University/Notebook
