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Team:Warsaw/Calendar-Main/24 June 2008
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MKrzyszton (Talk | contribs) (New page: {{WarNotebook}} <!-- do not edit above me! --> <p> 1. PCR - translation fusion: AID + T7 RNA-polimerase Primers: AIDlNrH and T7pXbSal Template DNA: AID for translation fusion and T7 R...) |
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| - | 1. PCR - translation fusion: AID + T7 RNA-polimerase | + | 1. PCR - translation fusion: AID + T7 RNA-polimerase <br> |
| - | Primers: AIDlNrH and T7pXbSal | + | Primers: AIDlNrH and T7pXbSal<br> |
| - | Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion | + | Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion<br> |
| - | Annealing temperature: 55 °C | + | Annealing temperature: 55 °C<br> |
| - | Annealing time: 4 minutes | + | Annealing time: 4 minutes<br> |
| - | two reapeats: 20 and 25 cycles | + | two reapeats: 20 and 25 cycles<br> |
2. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase) </p> | 2. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase) </p> | ||
Revision as of 19:48, 22 September 2008
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1. PCR - translation fusion: AID + T7 RNA-polimerase
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