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Team:Warsaw/Calendar-Main/25 June 2008
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1. Digestation of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI. <br> | 1. Digestation of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI. <br> | ||
2. Clean-up of digested PCR product. <br> | 2. Clean-up of digested PCR product. <br> | ||
| - | 3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (trzeba dodac wielkosc) | + | 3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (trzeba dodac wielkosc)<br> |
| + | 4. Overnight ligation of purified DNA fragments | ||
</p> | </p> | ||
Revision as of 20:18, 22 September 2008
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Preparation of pMPMT5-AID+AIDT7 construct
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