From 2008.igem.org
(Difference between revisions)
|
|
| Line 4: |
Line 4: |
| | | | |
| | | | |
| - | <p>'''Preparation of OmpA protein fusions'''<br> | + | <p></p> |
| - | 1. PCR on pBD (jaki numerek?) plasmid with ompaL_N and ompaP_link primers (15 cycles, annealing length 45s, annealing temperature 63 degrees)<br>
| + | |
| - | 2. PCR on pUC19 plasmid with alphaL_link and alphaP_XB primers (20 cycles, annealing length 45s, annealing temperature 63 degrees)<br>
| + | |
| - | 3. PCR on pUC19 plasmid with omegaL_link and omegaP_EPB primers (20 cycles, annealing length 30s, annealing temperature 58 degrees)<br>
| + | |
| - | As a result we got three PCR products: OmpA_linker and two fragments of TEM1 beta-lactamese: linker_alpha and linker_omega. <br>
| + | |
| - | 4. Gel electrphoresis of PCR products and gel-out of proper bands</p>
| + | |
| | | | |
| | | | |
Revision as of 18:39, 23 September 2008
"
