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Team:Warsaw/Calendar-Main/30 June 2008
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| - | <p>'''Optimisation of primers from 19.06'''<br></p> | + | <p>'''Optimisation of primers from 19.06'''<br> |
| + | 1. PCR on pBD (jaki numerek?) plasmid with ompaL_N and ompaP_link primers (annealing length 45s)<br> | ||
| + | 2. PCR on pUC19 plasmid with alphaL_link and alphaP_XB primers (annealing length 45s)<br> | ||
| + | 3. PCR on pUC19 plasmid with omegaL_link and omegaP_EPB primers (annealing length 30s)<br> | ||
| + | Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees</p> | ||
Revision as of 18:50, 23 September 2008
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Optimisation of primers from 19.06
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