Team:Warsaw/Calendar-Main/5 July 2008
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<p>'''Preparation of constructs with OmpA protein fusions'''<br> | <p>'''Preparation of constructs with OmpA protein fusions'''<br> | ||
1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) <br> | 1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) <br> | ||
- | 2. </p> | + | 2. Control digest of plasmids with NotI and BamHI<br> |
+ | 3. Gel electrophoresis <br> | ||
+ | </p> | ||
Revision as of 19:47, 23 September 2008