Team:Warsaw/Calendar-Main/25 June 2008
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<p>'''Preparation of pMPMT5-AID+AIDT7 construct''' <br> | <p>'''Preparation of pMPMT5-AID+AIDT7 construct''' <br> | ||
- | 1. Digestation of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI | + | 1. Digestation of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI, dephosphorylation of pMPMT5-AID with CIAP <br> |
2. Clean-up of digested PCR product. <br> | 2. Clean-up of digested PCR product. <br> | ||
3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (trzeba dodac wielkosc)<br> | 3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (trzeba dodac wielkosc)<br> |
Revision as of 20:58, 23 September 2008