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Template:Team:UC Berkeley/Notebook/MJV construction/corrected assembly vector
From 2008.igem.org
(Difference between revisions)
(New page: '''Removing attR2 point mutation in pBjh1601CA''' <pre> PCR mv042F/mv042R on pBbjh1601CA (3861bp, gp, BglII/MfeI) Sub in pBca1256-attR2 (EcoRI/BamHI/DpnI, 2472+151, S)...) |
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'''Removing attR2 point mutation in pBjh1601CA''' | '''Removing attR2 point mutation in pBjh1601CA''' | ||
<pre> | <pre> | ||
| - | PCR mv042F/mv042R on | + | PCR mv042F/mv042R on pBjh1601CA (3861bp, gp, BglII/MfeI) |
Sub in pBca1256-attR2 (EcoRI/BamHI/DpnI, 2472+151, S) | Sub in pBca1256-attR2 (EcoRI/BamHI/DpnI, 2472+151, S) | ||
| - | Product is | + | Product is pK112128CA |
----------------------------------------------- | ----------------------------------------------- | ||
mv042F Forward oligo for pBjh1600CA to patch attR2 (BglII) | mv042F Forward oligo for pBjh1600CA to patch attR2 (BglII) | ||
Latest revision as of 05:24, 26 September 2008
Removing attR2 point mutation in pBjh1601CA
PCR mv042F/mv042R on pBjh1601CA (3861bp, gp, BglII/MfeI) Sub in pBca1256-attR2 (EcoRI/BamHI/DpnI, 2472+151, S) Product is pK112128CA ----------------------------------------------- mv042F Forward oligo for pBjh1600CA to patch attR2 (BglII) gcagtAGATCTcttacAGATCCcggttatcc mv042R Reverse oligo for Bjh1600CA to patch attR2 (MfeI) gcagtCAATTGctgcagactggctgtg
