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Team:Warsaw/Calendar-Main/19 May 2008
From 2008.igem.org
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Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion | Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion | ||
Revision as of 13:43, 26 September 2008
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1. Gel electrophoresis (yesterday PCR's) and DNA isolation from proper bands. 2. PCR - translation fusion: AID + T7 RNA-polimerase Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase)
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