Team:Warsaw/Calendar-Main/10 July 2008

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2. Control digestation of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega). We didn't obtain pCACYC177 + OmpA_alpha probably because mistake in plating. <br>
2. Control digestation of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega). We didn't obtain pCACYC177 + OmpA_alpha probably because mistake in plating. <br>
3. Ligation of pACYC177 and OmpA_alpha (1 hr)<br>
3. Ligation of pACYC177 and OmpA_alpha (1 hr)<br>
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4. Transformation of E. coli TOP10 strain with ligation pACYC177 and OmpA_alpha.
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4. Transformation of E. coli TOP10 strain with ligation pACYC177 and OmpA_alpha. <br>
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2. Transformants plating on LB + kanamycin
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5. Transformants plating on LB + kanamycin.
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Preparation of constructs with OmpA protein fusions
1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digestation of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega). We didn't obtain pCACYC177 + OmpA_alpha probably because mistake in plating.
3. Ligation of pACYC177 and OmpA_alpha (1 hr)
4. Transformation of E. coli TOP10 strain with ligation pACYC177 and OmpA_alpha.
5. Transformants plating on LB + kanamycin.



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