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Team:Warsaw/Calendar-Main/2 June 2008
From 2008.igem.org
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<h4>PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase</h4><p> | <h4>PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase</h4><p> | ||
| - | Primers: AIDlNrH and T7pXbSal<br> | + | Primers: |
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| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a><br></html> | ||
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)<br> | Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)<br> | ||
Revision as of 13:52, 26 September 2008
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Piotr: Transformation of E.coli TOP10 with pZC320. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5) Annealing temperature: 55°C Time: 4 minutes Number of cycles: 20
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