Wiki/Team:Warsaw/primers

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<h4>Sequencing of pMPM-T5</h4>
<h4>Sequencing of pMPM-T5</h4>
<pre>
<pre>
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<a name="pMPMT5seqF">pMPMT5seqF</a>   5' CTACTGTTTCTCCATACCCG  3'
+
<a name="pMPMT5seqF">pMPMT5seqF</a> 5' CTACTGTTTCTCCATACCCG  3'
-
<a name="pMPMT5seqR">pMPMT5seqR</a>   5' GAAAATCTTCTCTCATCCGC 3'
+
<a name="pMPMT5seqR">pMPMT5seqR</a> 5' GAAAATCTTCTCTCATCCGC 3'
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<a name="AIDseqP">AIDseqP</a>     5' GCTTCGGCGCATCCTTTTG 3'
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<a name="AIDseqP">AIDseqP</a>     5' GCTTCGGCGCATCCTTTTG 3'
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</pre>
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 +
<h4>Sequencing of β-galactosidase from pZC320</h4>
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<pre>
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<a name="pZCseqL">pZCseqL</a>    5' CTTGACCCGCAGTTGCAAAC 3'
 +
<a name="pZCseqP">pZCseqP</a>    5' CCCAACTGATCTTCAGCATC 3'
</pre>
</pre>
<br><br><br><br>
<br><br><br><br>

Revision as of 13:57, 26 September 2008

Gallery Bricks Notebook Team Project Home

 

Primers

One-armed bandit preparation

AID and T7 cloning

T7lLinkB   5' CGGATCCGGCGGTGGCGGTAGTGGAGGAGGTGGGTCTGGAGGCGGAGGGAACACGATTAACATCGCTAAGAAC  3'
T7lRBSHi   5' CACAAGCTTAGGAGGTGTACTAGATGAACACGATTAACATCGCTAAG  3'
T7pXbSal   5' TCTGTCGACTCTAGATTACGCGAACGCGAAGTCC  3'
AIDlNrH    5' CGTAAGCTTAGGAGGTGTAGACCATGGACAGCCTCTTGATG  3'
AIDpLinB   5' CCACTACCGCCACCGCCGGATCCGCCACCTCCGCTGCCTCCACCACCAAGTCCCAAAGTACGAAATGC  3'

Sequencing of pMPM-T5

pMPMT5seqF  5' CTACTGTTTCTCCATACCCG  3'
pMPMT5seqR  5' GAAAATCTTCTCTCATCCGC 3'
AIDseqP     5' GCTTCGGCGCATCCTTTTG 3'

Sequencing of β-galactosidase from pZC320

pZCseqL     5' CTTGACCCGCAGTTGCAAAC 3'
pZCseqP     5' CCCAACTGATCTTCAGCATC 3'




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