Team:Warsaw/Calendar-Main/16 June 2008

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(Difference between revisions)
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Number of cycles:
Number of cycles:
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<p>DNA gel electrophoresis of PCR products.<br>
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<p>DNA gel electrophoresis of PCR products.</p>
-
<p>Gel-out of proper products (~1200 bp).<br>
+
<p>Gel-out of proper products (~1200 bp).</p>
<p>Sequencing of proper fragments using primer PZCseqL.</p>
<p>Sequencing of proper fragments using primer PZCseqL.</p>

Revision as of 14:09, 26 September 2008

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Colony PCR
Template: DNA isolated from white colonies
Primers: PZCseqL and PZCseqR
Annealing time: Number of cycles: <p>DNA gel electrophoresis of PCR products.

Gel-out of proper products (~1200 bp).

Sequencing of proper fragments using primer PZCseqL.

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