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Team:Warsaw/Calendar-Main/2 June 2008
From 2008.igem.org
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| - | <p>Piotr: Transformation of E.coli TOP10 with pZC320.</p> | + | <p>Piotr: <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with pZC320.</p> |
<p>Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG</p> | <p>Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG</p> | ||
Revision as of 15:00, 26 September 2008
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Piotr: <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of E.coli TOP10 with pZC320. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5) Annealing temperature: 55°C Time: 4 minutes Number of cycles: 20
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