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Team:Warsaw/Calendar-Main/12 May 2008
From 2008.igem.org
(Difference between revisions)
(New page: {{WarNotebook}} 1. Restriction digest of pTrc99A-AID with HindIII and NcoI. 2. DNA electrophoresis and isolation of DNA encoding AID from proper band. 3. Restriction digest of pMPM-T5 w...) |
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| - | 1. Restriction digest of pTrc99A-AID with HindIII and NcoI. | + | <p>1. Restriction digest of pTrc99A-AID with HindIII and NcoI.</p> |
| - | 2. DNA electrophoresis and isolation of DNA encoding AID from proper band. | + | <p>2. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band.</p> |
| - | 3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP). | + | <p>3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).</p> |
| - | 4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band. | + | <p>4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band.</p> |
| - | 5. DNA ligation (pMPM-T5+AID vector) | + | <p>5. DNA ligation (pMPM-T5+AID vector)</p> |
<html><div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div></html> | <html><div align="center"><img src="http://openwetware.org/images/e/e1/UW_AID.jpg" height="340"/></div></html> | ||
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{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Revision as of 15:41, 26 September 2008
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1. Restriction digest of pTrc99A-AID with HindIII and NcoI. 2. DNA electrophoresis and isolation of DNA encoding AID from proper band. 3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP). 4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band. 5. DNA ligation (pMPM-T5+AID vector)  </html>
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