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Team:Warsaw/Calendar-Main/12 May 2008
From 2008.igem.org
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<p>3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).</p> | <p>3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).</p> | ||
| - | <p>4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band.</p> | + | <p>4. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of pMPM-T5 vector from proper band.</p> |
<p>5. DNA ligation (pMPM-T5+AID vector)</p> | <p>5. DNA ligation (pMPM-T5+AID vector)</p> | ||
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1. Restriction digest of pTrc99A-AID with HindIII and NcoI. 2. DNA electrophoresis and isolation of DNA encoding AID from proper band. 3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP). 4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band. 5. DNA ligation (pMPM-T5+AID vector)  </html>
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