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Team:Warsaw/Calendar-Main/19 May 2008
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Michał K.: 1. Gel electrophoresis (friday PCR's) and DNA isolation from proper bands. 2. PCR - translation fusion: AID + T7 RNA-polimerase Primers: AIDlNrH and T7pXbSalTemplate DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase)
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