Team:NTU-Singapore/Notebook/6 June 2008
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(Difference between revisions)
(New page: =Friday 6 June= ==Lab details (to be edited)== Hung,Choonkit: *Morning: **Take out the PCR samples **Prepare digestion solutions for T7, GFP producer, RBS 0032 and 0034, Immunity 1 and 2, ...) |
(→Lab details (to be edited)) |
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Line 7: | Line 7: | ||
*Afternoon: | *Afternoon: | ||
**Gel electrophoresis. | **Gel electrophoresis. | ||
- | **Inoculation of GFP producer and RBS 0032 | + | **Inoculation of GFP producer, RBS 0034 and RBS 0032 as the gel electrophoresis showed wrong results. |
+ | **Transformation of LacI-GFP plasmid into top10 competent cells for characterization of LacI promoter next week. |
Revision as of 03:06, 9 June 2008
Friday 6 June
Lab details (to be edited)
Hung,Choonkit:
- Morning:
- Take out the PCR samples
- Prepare digestion solutions for T7, GFP producer, RBS 0032 and 0034, Immunity 1 and 2, CoE7, LsrA 1 and 2.
- Afternoon:
- Gel electrophoresis.
- Inoculation of GFP producer, RBS 0034 and RBS 0032 as the gel electrophoresis showed wrong results.
- Transformation of LacI-GFP plasmid into top10 competent cells for characterization of LacI promoter next week.