Team:NTU-Singapore/Notebook/6 June 2008

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(Difference between revisions)
(New page: =Friday 6 June= ==Lab details (to be edited)== Hung,Choonkit: *Morning: **Take out the PCR samples **Prepare digestion solutions for T7, GFP producer, RBS 0032 and 0034, Immunity 1 and 2, ...)
(Lab details (to be edited))
Line 7: Line 7:
*Afternoon:
*Afternoon:
**Gel electrophoresis.
**Gel electrophoresis.
-
**Inoculation of GFP producer and RBS 0032
+
**Inoculation of GFP producer, RBS 0034 and RBS 0032 as the gel electrophoresis showed wrong results.
 +
**Transformation of LacI-GFP plasmid into top10 competent cells for characterization of LacI promoter next week.

Revision as of 03:06, 9 June 2008

Friday 6 June

Lab details (to be edited)

Hung,Choonkit:

  • Morning:
    • Take out the PCR samples
    • Prepare digestion solutions for T7, GFP producer, RBS 0032 and 0034, Immunity 1 and 2, CoE7, LsrA 1 and 2.
  • Afternoon:
    • Gel electrophoresis.
    • Inoculation of GFP producer, RBS 0034 and RBS 0032 as the gel electrophoresis showed wrong results.
    • Transformation of LacI-GFP plasmid into top10 competent cells for characterization of LacI promoter next week.