Team:Warsaw/Calendar-Main/9 July 2008
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2. Confirmed transformant colonies inoculated to liquid LB with kanamycin</p> | 2. Confirmed transformant colonies inoculated to liquid LB with kanamycin</p> | ||
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+ | <p>'''Preparation of construct pKS with A protein''' <br> | ||
+ | 1. Colony PCR on colonies from plates with transformations pKS-A <br> | ||
+ | Primers used: | ||
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+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> | ||
+ | </html><br> | ||
+ | 2. Confirmed transformant colonies inoculated to liquid LB with ampicillin</p> | ||
Revision as of 11:50, 27 September 2008
Preparation of constructs with OmpA protein fusions
Preparation of construct pKS with A protein
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