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Team:Warsaw/Calendar-Main/16 May 2008
From 2008.igem.org
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a> | ||
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| + | Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> | ||
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Annealing temperature: 55 °C | Annealing temperature: 55 °C | ||
Revision as of 15:32, 27 September 2008
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Michał K.: 1. PCR - AID for translation fusion Primers:
AIDlNrH
AIDpLinB
Annealing time: 60 sec 20 cycles
Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles 3. PCR - T7 RNA-polimerase for transcription fusion Primers:
Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles
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