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Team:Warsaw/Calendar-Main/12 May 2008
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<p>Michał K.:</p> | <p>Michał K.:</p> | ||
| - | <p>1. Restriction | + | <p>1. Restriction digestion of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> with HindIII and NcoI (1x Tango buffer).</p> |
<p>2. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band (600 bp).</p> | <p>2. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band (600 bp).</p> | ||
| - | <p>3. Restriction | + | <p>3. Restriction digestion of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with HindIII and NcoI (1x Tango buffer), use of alkaline phosphatase (CIAP).</p> |
<p>4. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp).</p> | <p>4. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> vector from proper band (4250 bp).</p> | ||
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Michał K.: 1. Restriction digestion of pTrc99A-AID with HindIII and NcoI (1x Tango buffer). 2. DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp). 3. Restriction digestion of pMPM-T5 with HindIII and NcoI (1x Tango buffer), use of alkaline phosphatase (CIAP). 4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp). 5. Overnight ligation of isolated DNA fragments.  </html>
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