Team:Warsaw/Calendar-Main/15 May 2008

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<p>1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID.</p>
<p>1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID.</p>
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<p>2. Restriction digest of plasmid with HindIII and NcoI - construct control.</p>
+
<p>2. Restriction digestion of plasmid with HindIII and NcoI (1xTango buffer) - construct control.</p>
-
<p>3. Gel - choice of proper clones.</p>
+
<p>3. Gel electrophoresis - choice of proper clones.</p>

Revision as of 23:03, 28 September 2008

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Michał K.

1. Isolation of hypothetical pMPM-T5+AID.

2. Restriction digestion of plasmid with HindIII and NcoI (1xTango buffer) - construct control.

3. Gel electrophoresis - choice of proper clones.