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Team:Warsaw/Calendar-Main/16 May 2008
From 2008.igem.org
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Annealing temperature: 55 °C | Annealing temperature: 55 °C | ||
| - | + | Elongation time: 60 sec | |
20 cycles | 20 cycles | ||
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Annealing temperature: 62 °C | Annealing temperature: 62 °C | ||
| - | + | Elongation time: 4 minutes | |
20 cycles<br> | 20 cycles<br> | ||
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Annealing temperature: 62 °C | Annealing temperature: 62 °C | ||
| - | + | Elongation time: 4 minutes | |
20 cycles | 20 cycles | ||
Revision as of 23:10, 28 September 2008
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Michał K.: 1. PCR - AID for translation fusion Primers:
AIDlNrH
AIDpLinB
Elongation time: 60 sec 20 cycles
Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles 3. PCR - T7 RNA-polimerase for transcription fusion Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles
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