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Team:Warsaw/Calendar-Main/19 May 2008
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<p>Michał K.:</p> | <p>Michał K.:</p> | ||
| - | <p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008"> | + | <p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands.</p> |
<p>2. PCR - translation fusion: AID + T7 RNA-polymerase</p> | <p>2. PCR - translation fusion: AID + T7 RNA-polymerase</p> | ||
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Michał K.: 1. Gel electrophoresis (16 May PCR's) and DNA isolation from proper bands. 2. PCR - translation fusion: AID + T7 RNA-polymerase Primers: Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)
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