Team:Paris/Modeling/f1

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[[Image:f1a.jpg|thumb]] In the '''exponential phase of growth''', at the steady state, we have
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[[Image:f1a.jpg|thumb]]
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<center>[[Image:tetReqtot.jpg]]</center>
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[[Image:f1expr.jpg|center]]
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so, with
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and at steady-state, in the exponential phase of growth :
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<center>[[Image:f1expr.jpg]]</center>
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[[Image:PbadArab.jpg|center]]
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that gives, after reducing the expression
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<center>[[Image:f1exprredu.jpg]]</center>
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with the previous expression of [TetR]<sub>eq</sub>
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<center>[[Image:f1exprreduf0.jpg]]</center>
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so, finally, if we use a ''calculated'' value of [TetR] (by using the expression of &#131;0 for instance, or by introducing a value given by ''tetR'' after an other "caracterised promoter")
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<center>[[Image:f1exprCalc.jpg]]</center>
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Revision as of 17:20, 29 September 2008

F1a.jpg
F1expr.jpg

and at steady-state, in the exponential phase of growth :

PbadArab.jpg


param signification unit value
[expr(pTet)] expression rate of
pTet with RBS E0032 		nM.min-1 see "findparam"
need for 20 measures
γGFP dilution-degradation rate
of GFP(mut3b) 		min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 measures
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
βtet production rate of pTet with RBS E0032
β1 		nM.min-1
(Ktet/{coeftetR}) activation constant of pTet
K18 		nM
ntet complexation order of pTet
n18 		no dimension
KaTc complexation constant aTc-TetR
K17 		nM
naTc complexation order aTc-TetR
n17 		no dimension
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