Team:Warsaw/Calendar-Main/16 May 2008
From 2008.igem.org
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Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> | Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> | ||
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Annealing temperature: 55 °C | Annealing temperature: 55 °C | ||
Revision as of 22:01, 29 September 2008
Michał K.: 1. PCR - AID for translation fusion. Elongation time: 60 sec 20 cycles
Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles 3. PCR - T7 RNA-polimerase for transcription fusion. Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Elongation time: 4 minutes 20 cycles
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