"
Team:Warsaw/Calendar-Main/14 May 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| Line 19: | Line 19: | ||
20 cycles <br> | 20 cycles <br> | ||
| - | 3. Optimalization of conditions for PCR - T7 RNA polymerase for translational | + | 3. Optimalization of conditions for PCR - T7 RNA polymerase for translational fusion; annealing temerature gradient (from 62°C to 82°C). <br> |
| + | |||
| + | Primers: | ||
| + | |||
| + | <html> | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lLinkB">T7lLinkB</a> | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a> | ||
| + | <br> | ||
| + | </html> | ||
| + | |||
| + | Template DNA: E. coli Rosetta genomic DNA<br> | ||
| + | |||
| + | Elongation time: 4 minutes | ||
| + | <br> | ||
| + | |||
| + | 20 cycles<br></p> | ||
{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Revision as of 22:10, 29 September 2008
 |
|
||||||||
 |
|
||||||||
Michał K.: 1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).
|
|
