Team:Paris/Modeling/f1
From 2008.igem.org
(Difference between revisions)
Line 78: | Line 78: | ||
Also, this experiment will allow us to know the expression of ƒ1 : | Also, this experiment will allow us to know the expression of ƒ1 : | ||
- | [[ExprF1.jpg|center]] | + | [[Image:ExprF1.jpg|center]] |
Revision as of 23:32, 29 September 2008
The experience would give us
Thus, at steady-state and in the exponential phase of growth :
param | signification | unit | value |
[expr(pTet)] | expression rate of pTet with RBS E0032 | nM.min-1 | see "findparam" need for 20 measures |
γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 |
[GFP] | GFP concentration at steady-state | nM | need for 20 measures |
(fluorescence) | value of the observed fluorescence | au | need for 20 measures |
conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
param | signification corresponding parameters in the equations | unit | value |
βtet | production rate of pTet with RBS E0032 β1 | nM.min-1 | |
(Ktet/{coeftetR}) | activation constant of pTet K18 | nM | |
ntet | complexation order of pTet n18 | no dimension | |
KaTc | complexation constant aTc-TetR K17 | nM | |
naTc | complexation order aTc-TetR n17 | no dimension |
Also, this experiment will allow us to know the expression of 1 :