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Team:Warsaw/Calendar-Main/20 May 2008
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<p>Michał K: <br> | <p>Michał K: <br> | ||
| - | 1. PCR - translation fusion: AID + T7 RNA-polymerase</p> | + | 1. PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60 dgr;C - 80 dgr; C).</p> |
<p>Primers: </p> | <p>Primers: </p> | ||
Revision as of 21:15, 30 September 2008
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Michał K: Primers: Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)
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