Team:Warsaw/Calendar-Main/20 May 2008

From 2008.igem.org

(Difference between revisions)
Line 5: Line 5:
<p>Michał K: <br>
<p>Michał K: <br>
-
1. PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60 dgr;C - 80 dgr; C).</p>
+
1. PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60&deg;C - 80&deg;C).</p>
<p>Primers: </p>
<p>Primers: </p>

Revision as of 21:17, 30 September 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Michał K:
1. PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60°C - 80°C).

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)



Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/20_May_2008"