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Team:Warsaw/Calendar-Main/20 May 2008
From 2008.igem.org
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></p> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></p> | ||
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Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion | Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion | ||
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Annealing time: 4 minutes | Annealing time: 4 minutes | ||
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3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase) | 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase) | ||
Revision as of 21:18, 30 September 2008
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Michał K: Primers: Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 35 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)
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