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Team:Warsaw/Calendar-Main/20 May 2008
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and | ||
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></p> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></p> | ||
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| + | Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br> | ||
| - | + | Annealing temperature: 55 °C <br> | |
| - | Annealing | + | Annealing time: 4 minutes <br> |
| - | + | 35 cycles <br> | |
| - | + | ||
| - | 35 cycles< | + | |
3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase) | 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase) | ||
Revision as of 21:19, 30 September 2008
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Michał K: Primers: Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusionAnnealing temperature: 55 °C Annealing time: 4 minutes 35 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)
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