Team:Warsaw/Calendar-Main/22 May 2008
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20 cycles <br> | 20 cycles <br> | ||
+ | 2. PCR - translation fusion: AID + T7 RNA-polymerase</p> | ||
+ | <p>Primers: </p> | ||
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+ | <p><html> | ||
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and | ||
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></html></p> | ||
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+ | Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br> | ||
+ | |||
+ | Annealing temperature : 60°C <br> | ||
+ | Annealing time: 4 minutes <br> | ||
+ | |||
+ | 20 cycles <br> | ||
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Revision as of 22:02, 30 September 2008