Team:Warsaw/Calendar-Main/21 May 2008

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<p>Michał K: <br>
<p>Michał K: <br>
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1. PCR - transcription fusion: AID + T7 RNA-polymerase - optimalization (temperature gradient 60&deg;C - 80&deg;C).</p>
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1. <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - transcription fusion: AID + T7 RNA-polymerase - optimalization (temperature gradient 60&deg;C - 80&deg;C).</p>
<p>Primers: </p>
<p>Primers: </p>

Revision as of 22:23, 30 September 2008

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Michał K:
1. PCR - transcription fusion: AID + T7 RNA-polymerase - optimalization (temperature gradient 60°C - 80°C).

Primers:

AIDlNrH and T7pXbSal


Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion


Annealing time: 4 minutes

35 cycles

2. Gel electrophoresis.



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