Alberta NINT/5 June 2008

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(Difference between revisions)
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               Performed Ligation of K102001+E0433=K102003 and K102001+null=K102005.   
               Performed Ligation of K102001+E0433=K102003 and K102001+null=K102005.   
               Transformed bacteria and plated them.
               Transformed bacteria and plated them.
 +
 +
lab work (SD):
 +
 +
              Inoculated 6 LB + amp50 culture tubes with colonies from K102009 plate (04/06/08).
 +
              Incubated at 37 C, shaking, overnight.
 +
              <br><br>
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              Plates K102008 and K102008 negative control had >100 colonies each.
 +
              Re-try digest using NEB 2 buffer.  Purified using MinElute PCR purification standard protocol.
 +
              Ligated K102002 /B+N with T/A 0 /B+N.

Revision as of 20:11, 9 June 2008

lab work (JD): 

             Purified gel band of K102001/PstI and SpeI from yesterday.  
             Collected quantitative data on K102001, E0433, and null output.  
             Performed Ligation of K102001+E0433=K102003 and K102001+null=K102005.  
              Transformed bacteria and plated them.

lab work (SD): 

             Inoculated 6 LB + amp50 culture tubes with colonies from K102009 plate (04/06/08).
             Incubated at 37 C, shaking, overnight.
             


             Plates K102008 and K102008 negative control had >100 colonies each.
             Re-try digest using NEB 2 buffer.  Purified using MinElute PCR purification standard protocol.
             Ligated K102002 /B+N with T/A 0 /B+N.
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