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Team:Warsaw/Calendar-Main/2 June 2008
From 2008.igem.org
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1.<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with pZC320.</p> | 1.<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> TOP10 with pZC320.</p> | ||
| - | <p>2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</p> | + | <p>2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</html></p> |
Michał K.: <br> | Michał K.: <br> | ||
Revision as of 13:35, 1 October 2008
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Piotr: 2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.</p> Michał K.: PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a> Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5) Annealing temperature: 55°C Time: 4 minutes Number of cycles: 20
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