Team:Warsaw/Calendar-Main/25 June 2008

From 2008.igem.org

(Difference between revisions)
Line 7: Line 7:
1. Digestion of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI, dephosphorylation of pMPMT5-AID with CIAP <br>
1. Digestion of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI, dephosphorylation of pMPMT5-AID with CIAP <br>
2. Clean-up of digested PCR product. <br>
2. Clean-up of digested PCR product. <br>
-
3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (trzeba dodac wielkosc)<br>
+
3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (4800 bp)<br>
4. Overnight ligation of purified DNA fragments
4. Overnight ligation of purified DNA fragments
</p>
</p>

Revision as of 14:59, 1 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Preparation of pMPMT5-AID+AIDT7 construct
1. Digestion of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI, dephosphorylation of pMPMT5-AID with CIAP
2. Clean-up of digested PCR product.
3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (4800 bp)
4. Overnight ligation of purified DNA fragments



Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008"