Team:Warsaw/Calendar-Main/31 July 2008

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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI ">AL+SacI</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI ">AL+SacI</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> </html></p>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> </html></p>
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Elongation time: 30s <br>
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- optimalization of annealing temperature (gradient from 55&deg;C to 75&deg;C).
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- opimalization of number of cycles(15, 20, 25, 30, 35)

Revision as of 15:10, 1 October 2008

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1. Optimalization of PCR to obtain trunced A protein DNA fragment
Primers: AL+SacI AP+NotI

Elongation time: 30s

- optimalization of annealing temperature (gradient from 55°C to 75°C). - opimalization of number of cycles(15, 20, 25, 30, 35)



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